GSM6102801: ChIP-seq-10-PAF1-SPT6AID-Veh-2h; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
PAF1
Cell type
Cell type Class
Digestive tract
Cell type
DLD-1
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
DLD-1
cell line
DLD-1
cell type
Colorectal adenocarcinoma
genotype
SPT6-AID OsTIR1
treatment
Vehcle 2h
chip antibody
PAF1 (Cell signaling # 12883)
Sequenced DNA Library
library_name
GSM6102801
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq in human DLD1 cells, ~30 million cells were crosslinked with 1% paraformaldehyde in PBS for 10 min at r.t. Spike-in mouse embryonic fibroblast fixed cells were added to samples. Chromatin was sonicated with the Covaris E220. Immunoprecipitations were carried out with antibodies and Dynabeads Protein G. For ChIP-seq, DNA libraries were prepared by the HTP Library Preparation Kit for Illimina (KAPA).